Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGINIE--NASFGLTNCAERTAFFKAVSEG--EKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPDMPVTLVGDNGVTKATTVRALLPYAFTEKDL- 1JTK Chain:A ((1-131)) --MNRQELITEALKARDMAYAPYSKFQVGAALLTKDGKVYRGCNIENA--AYSMCNCAERTALFKAVSEGDT--EFQMLAVAADTPGPVSPCGACRQVISELCTKDVIVVLTNLQGQIKEMTVEELLPGAFSSEDLH

General information: TITO was launched using: RESULT: Template: 1JTK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 664 -41535 -62.55 -329.64 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -62.55 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.541

(partial model without unconserved sides chains): PDB file : Tito_1JTK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JTK-query.scw PDB file : Tito_Scwrl_1JTK.pdb: