Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNPATQDAAAAEEESPVVCTAKKYIFDLHSYAWKESDCLIRIPRPHKGMGHGGMRVCYAVEDVDEEGAGTPMVAKMFRRNISDVVEKD-YFNEGAAQCMCEEFANNFNRIHLTNIHKP--NISFLQCYVVRIPRDSIPVASQKKRTGFFSYKTQDTGEVMFVMEPMLSGKFTKYNSNYGETYREDKKAALTSSEAKRRTEVFEAAEAFSHFTLVESGGSMLVCDLQGVNDFLTDPQIHTEDGKGLGMGNMGQEGIDKWIEKHECNEICRALGLQPLLGAVPSSTACAKEENLNLGLRAQLQSQNPVRLRDLVPLTKPLEEMTEAERIEYALKVSRLTD 4ZME Chain:A ((105-281)) --------------------------------------------------------------------GTKFVLKLYKK---QQASRELYFEDVKMQMVCRDWGNKFNQ------KKPPKKIEFLMSWVVEL-IDRSPSSNGQ--------------PILCSIEPLLVGEFKKNNSNYG--------AVLTN----RST-----PQAFSHFTYELSNKQMIVVDIQGVDDLYT-PQIHTPDGKGFGLGNLGKAGINKFITTHKCNAVCALLDLDVKLGGVLSG-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4ZME.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 791 5996 7.58 35.27 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 7.58 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.563

(partial model without unconserved sides chains): PDB file : Tito_4ZME.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZME-query.scw PDB file : Tito_Scwrl_4ZME.pdb: