Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSGLILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGTMDMVVERNAYGRQLGSFYTEAECKGVGKIPMT--FIRGPIISSVGEGVEILAIVNNQIVAAQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS 2NV2 Chain:B ((2-189)) LTIGVLGLQGAVREHIHAIEACGAAGLVVKRPEQL----NEVDGLILPGGESTTMRRLIDTYQFMEPLREFAAQGKPMFGTCAGLIILAKEIAGSDNPHLGLLNVVVERNSFGRQVDSFEADLTIKGLDE-PFTGVFIRAPHILEAGENVEVLSEHNGRIVAAKQGQFLGCSFNPELTEDHRVTQLFVEMVEE--

General information: TITO was launched using: RESULT: Template: 2NV2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1117 -99711 -89.27 -536.08 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -89.27 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.644

(partial model without unconserved sides chains): PDB file : Tito_2NV2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NV2-query.scw PDB file : Tito_Scwrl_2NV2.pdb: