Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIALFYMGGTFGCIGEPLAPMPYDQFLPQLEKVIPPHLTVDCFAAPNIVDSSACTAPDWLRLIQRIQQLQLEGYQHFVVIHGTDTLSYAAATLARFLGQS-CHIVITGSQYPLLNIQGDNTREFTDAIENLYLALEQVIALLVGAYLAFHHQVFHAQTALKTHTTELDAFSGLS-SNVEFTPQQNELIVQDAQIEKAASFQLLNWMMQPIATQHLVQQL-----RHLLPAPPHFLVLQGFGTGNIAVNDEFLATLDELYTRGCVPILATQVTFGGID-QRYAISTWAKTAKIVINDAHSHADLYAKALQIYLKYPTPEQWLNHWNENLH 1WNF Chain:A ((50-285)) --------------------------------------------------VDSTLIQPSDWERLAKEIEKEVWE-YDGIVITHGTDTMAYSASMLSFMLRNPPIPIVLTGSMLPI-------TEKNSDAPFNLRTALEFVKLGIRGIYIAFNGKVMLGVRASKIRSMGFDAFESINYPNVAEIKDDKLRILHIPDFYGDEFFSDIKYEPKVLVIK-LIPGLSGDIVREALRLGYKGIILEGYGVGGIPYRGTDLFEVVSSISKRIPVVLTTQAIYDGVDLQRYKVGRIALEAGVI------------------------------------

General information: TITO was launched using: RESULT: Template: 1WNF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1022 -97316 -95.22 -426.82 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -95.22 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.218

(partial model without unconserved sides chains): PDB file : Tito_1WNF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WNF-query.scw PDB file : Tito_Scwrl_1WNF.pdb: