Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALTNADRAEIIAKFARAENDTGSPEVQVALLTAQINDLQGHFKAHKHDHHSRRGLIRMVNQRRKLLDYLNGKDHERYTALIGALGLRR 5MY1 Chain:O ((2-89)) -SLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFAEHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTRLIERLGLRR

General information: TITO was launched using: RESULT: Template: 5MY1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain O - contact count / total energy / energy per contact / energy per residue : 233 -29651 -127.26 -336.94 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain O : 0.90 3D Compatibility (PKB) : -127.26 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.676

(partial model without unconserved sides chains): PDB file : Tito_5MY1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MY1-query.scw PDB file : Tito_Scwrl_5MY1.pdb: