Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIAQALAIRGEPDSYERQENAWLLEHFLKINSLELKFRLEQELTAQQEQDYLAGLERIQNGEPLAYVTGSQPFWTLDLKVTSDTLVPRPDT-EVLVETVLNLNLPKNANIVDLGTGTGAIALALASERPDWFVTATDIYAPTLEVAKENAQTHGLHHVKFACGVWYEALEP--QQF---DLIVSNPPYIDPEDEHMQALATEPRRALVADHHGLADIEIIIAQGKNWLKPQGWIALEHGYDQGQAVRGIFAEHGFSEIRTIQDYGRNDRVTLASLLIK 1SG9 Chain:A ((58-226)) -------------------------------------------VSPTEEKRILELVEKRASGYPLHYILGEKEFMGLSFLVEEGVFVPRPETEELVELALELIRKYGIKTVADIGTGSGAIGVSVA-KFSDAIVFATDVSSKAVEIARKNAERHGVSDRFFVR--KGEFLEPFKEKFASIEMILSNPPYVKSSAHLPKDVLFEPPEALFGGEDGL----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1SG9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 755 -18232 -24.15 -111.85 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -24.15 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.490

(partial model without unconserved sides chains): PDB file : Tito_1SG9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SG9-query.scw PDB file : Tito_Scwrl_1SG9.pdb: