Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLELDQDGHLVDYTIWNPEVAQELAKSLDL-ELTDWHFEVLAAVRQFYQQFGHSPATRPLIK---FLMKTVSPDINNAVLQQKFNTGLVARHLSRLAGIPKPANCL 2V4J Chain:C ((10-105)) -SFEVDEDGFLLRFDDWCPEWVEYVKESEGISDISPDHQKIIDFLQDYYKKNGIAPMVRILSKNTGFKLKEV---------YELFPSG-PGKGACKMAGLPKPTGCV

General information: TITO was launched using: RESULT: Template: 2V4J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 356 -19089 -53.62 -207.49 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.73 3D Compatibility (PKB) : -53.62 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.641

(partial model without unconserved sides chains): PDB file : Tito_2V4J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2V4J-query.scw PDB file : Tito_Scwrl_2V4J.pdb: