Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNFLPAEEQLALIQRGTHEIISEEDLLKKLKENRPLKIKAGFDPTAPDLHLGHTVLINKLKTFQDLGHEVTFLIGDYTAMIGDPTGKSATRPPLSREQVEANAKTYQEQVFKILD----PNKTKVRFNSEWFNQKSAADLIQ-LASQQTVSRMLERDDFTKRYNNH-QPIAIHEFLYPLVQGYDSIALEAD----VELGGTDQTFNLLMGRTLQSRYGQESQVCITVPILEGLDGVNKMSKSLGNYIGV---FDAPGAMYQKVLSMPDSLIERYFDLLSFKSLDEIKALLDEIA-AGRNPQEVKRILALELVERFHDAEAAANAHKSAGNRITEGEVPADTPEVTISRGEFGGEIFIATILRVAGLNPNAAAAKDAVARGAVKVDWNVVDASFSVKENGTFIIQSGKKAIARVTFTD 1X8X Chain:A ((10-322)) ------------LQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVDKIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQRLNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGT-KFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSIEEINALEEEDKNSGKAPR-AQYVLAEQVTRLVHGEEGLQAAKR-------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1X8X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1443 -949 -0.66 -3.17 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -0.66 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.510

(partial model without unconserved sides chains): PDB file : Tito_1X8X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1X8X-query.scw PDB file : Tito_Scwrl_1X8X.pdb: