Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTNLAHKQEEDNVITLHPSTAKKKERTAMSDKFDKGYVMSSR-LYRNEVKPFLGDAARNVYAELEEYIS-----GF--NKESDFV--SYSQLQGKKIEGLEEHVRKLSTATVRAGLKQLIEYGVISIVATNPKL-GNKYKLNEISLVEHFSNKSTSETKALQKLNSTTLETKAQGTLETKDTIDIIYRYLIIDNLFNSLRSNKPLENHFFVYQENKKQVFLEQQKLEAEEKAKAEKERKDKVRKLSFDEVIKLTKTTFANLCDLELWEQYVAFRSQKANTKLTKNALNVIYKDLKAWGFQGSNQSLKTAISGNYLGLFAPKQQNHGFVNQSQASTRMSEIQELIAKEEAGNEQYGF 4PT7 Chain:C ((10-114)) -------------------------------ENYKERFYQIPKVFFTSENYKNLTNDMKIAYAILRDRLNLSIKNSWVDEDGNIYFVYSNEKLMEI---------LNCKKEKLTKIKKGLENDGLLIQKRRGLNKPNILYLMKPI--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4PT7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 242 11088 45.82 117.95 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain C : 0.59 3D Compatibility (PKB) : 45.82 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.404

(partial model without unconserved sides chains): PDB file : Tito_4PT7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PT7-query.scw PDB file : Tito_Scwrl_4PT7.pdb: