Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFLAEFNQAPPDQLKTLLNNCVHIPVWAEKIISERPYSSTSVLLGYAEQLSHTWSWQEIQAA--------LATHPKI-GERQAKKQLNAKEQNFSAQEQAGISLDE---QTQQALLQGNIEYEQKFGFIFLIKAAGLSSDEILKKLHQRLQNNLAIEKVIVHEQLAAIALLRLSQEVQA 2O8I Chain:A ((20-159)) ------------------------------FIAERAYDAGGAGLELTAKAVHGALCAQFRVASEAERLGVLRAHPDLAGKLAIAGELT--------------GLDRLSPQEHARFTQLNSAYTEKFGFPFIIAVKGLNRHDILSAFDTRIDNNAAQEFATATGQVEKIAWLRLA-----

General information: TITO was launched using: RESULT: Template: 2O8I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 342 -3574 -10.45 -30.29 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -10.45 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.454

(partial model without unconserved sides chains): PDB file : Tito_2O8I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2O8I-query.scw PDB file : Tito_Scwrl_2O8I.pdb: