Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNALTQELVELLTLEKLEENIYRGISRNLVGKRVFGGQVLGQALRAASYTTDRPAHSLHAYFLYGGDINAPIIYEVDRLRDGKSFVSRQVRAIQHGRVIFSAMVSFANPEEGLNYQH--PEPDYPAPEALKSESELKEGILNFVPENVRASFMRERHVEIRPIDPVNPFQPQPEAPFNAH--YIRTHDRIPKQLEDISLHQAIVAFYSDFTLMTTALRPHGLSYISPSLQCASIDHAIYFHRPLRADEWMLYDMEATVSAASRGLNFGRMW-QNGQLVCSTVQEGLMRLREIETQ 3CJY Chain:A ((35-258)) ---------------------------------LFGGASMALALDVAAETVGRPVVQGSLQFVSFTPLGSVLDLTVEVLQSGRTLAQARVAGTVDGRLVFHSGISLGM-REGFSARQWALAPPVPQPDNCPPCTTLPA-------QDDNARYL--EGIEVREAG-------GPEVPSGRTRLWLRRKDGAP-------LDAASLAMFADFLPIALG-RATGCSG--NSL-----DNSLRITG-AAAPGWCLCDMIIPSSASGFAQGQVTLWDQSGRLLATGAQSLLLK-------

General information: TITO was launched using: RESULT: Template: 3CJY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1152 -48762 -42.33 -224.71 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -42.33 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_3CJY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CJY-query.scw PDB file : Tito_Scwrl_3CJY.pdb: