Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNIYQFEAELLVGDIKQF--ADYKGKVLLIVNTASKCGFTPQ-FAGLEKLYEKYKDLGLEVLGFPCNQFGGQDPGSNKEIGTFCQRNYGVKFPMFAKVDVKGPEAHVIFRYLTREAKGILGSSTIKWNFTKFLVGKDGSVLNRYAPTTKPEALEADIEKALAS 2VUP Chain:A ((8-159)) ------FDFEVLDADHKPYNLVQHKGSPLLIYNVASKCGYTKGGYETATTLYNKYKSQGFTVLAFPCNQFGGQEPGNEEEIKEFVCTKFKAEFPIMAKINVNGENAHPLYEYMKKTKPGILKTKAIKWNFTSFLIDRDGVPVERFSPG----ASVKDIEKKL--

General information: TITO was launched using: RESULT: Template: 2VUP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 733 12705 17.33 85.27 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : 17.33 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.561

(partial model without unconserved sides chains): PDB file : Tito_2VUP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2VUP-query.scw PDB file : Tito_Scwrl_2VUP.pdb: