Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNSVQYLFSCLLGMTTFSVGITPTSAGQMYIYQDKNGSTLLTNRKSYDHSLKKVKVTYYPDSNIHSYSNWGTSEASVLPSYSKNKNAFDHIIKQAAQQHGVSEGLIKAVMHTESGFNVNVRSPVGAQGLMQLMPATARRF-----------NVSNAYDPQQNIFAGAKYLSWLLKRFNGNTQ-------MALAAYNAGEGNVDKYGGIPPFRETQDYVRRVTSRYQNLYSSGVGLSSFSNSSISAQAINQPAIPHSTSTQVSAQPIKYSSSRQIVTLPDGTYTDAPTGTYVTNNATAIAHIRIE 4CFO Chain:A ((177-288)) -------------------------------------------------------------------------------------------MVRQASRKYGVDESLILAIMQTQSSFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAMLNNVYLGGIDNPTSRRYAVITAYNGGAGSV------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4CFO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 394 -35102 -89.09 -373.42 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -89.09 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_4CFO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CFO-query.scw PDB file : Tito_Scwrl_4CFO.pdb: