Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIKFNMVAKKLALILCSLSFLQTSFAGFERFWIFSKDANTQVDDTWNALSEEEQRAVIKRYQSLKELPSNQSANLQQRMDWFNQLPDAEKQKMREAWQKMSTQERNDLRQRMLKASADERTAIREEYINKYLEH 1KGN Chain:A ((40-108)) -------------------------------FWLPEKIPVSNDIQSWNKMTPQEQLATMRVFTGLTLLDT-----IQGTVGAISLLPDAETMHEEAVYTNIAFME------------------------------

General information: TITO was launched using: RESULT: Template: 1KGN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 86 623 7.24 9.02 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 7.24 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.348

(partial model without unconserved sides chains): PDB file : Tito_1KGN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KGN-query.scw PDB file : Tito_Scwrl_1KGN.pdb: