Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASLRSSAIPVSDPASGLRITEIFY-SLQGEANASGLPTVFIRLTGCPLRCSYCDTTYSFEGGERLSLEHIIETAEKY----------QTPYICVTGGEPLAQPNCLILLQRLCDAGFDVSLETSGALDVSRVDPRVSKVLDLKTPTSGEEHRNLISN---LDHLTPRDQ---------IKFVICNREDYEWSKQQVEQYQLQTKVSTVWFSPAFAVEKGAVGLPRLARDMAQWILDDKLPVRFQL-------QLHKLLWNDESGR 5TGS Chain:A ((22-257)) --------------AKGIPVLEIFGPTIQGEGMVIGQKTMFVRTAGCDYSCSWCDSAFTWDGSAKKDIRWM--TAEEIFAELKDIGGDAFSHVTISGGNPALLKQLDAFIELLKENNIRAALETQGTVYQDWFTLIDDLTISPKPPSS-----KMVTNFQKLDHILTSLQENDRQHAVSLKVVIFNDEDLEFAKTVHKRYPGIPFYLQVGNDDV------SLIAHLLGKYEA---LVDKVAVDAELNLVRVLPQLHTLLW------

General information: TITO was launched using: RESULT: Template: 5TGS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 977 -19392 -19.85 -96.96 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -19.85 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.275

(partial model without unconserved sides chains): PDB file : Tito_5TGS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TGS-query.scw PDB file : Tito_Scwrl_5TGS.pdb: