Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFDHIAQPISYQHIELSFPVHLDIKRLDLVHPQISGNKFFKLKYNLLAAKEQGLSSILTFGGAYSNHIAATAYAAHLFGLKSIGIIRGEELAGKPLNPTLAKAQSLGMQLHFVSRHEY--RLRDEANYLKQL----QQQFPQTYIIPEGGTNELAVQG-----------CQEILSQYDLEQYDVICCAVGTGGTISGL-----------------IERSAAHQKVLGFSALKGDFLQQEIK---QWTKKQNWSLTDVYCWGGYAKTSPELFTFIENFEEQYTVPLEPIYTGKMMFGLFDLIKNNYFPANTRILAIHSGGLQADIRNRSHA 4D9E Chain:A ((51-334)) -----------------------IKRDDVTPIAMGGNKLRKLEFLVADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALL--ENPIGTTAENYLTNGNRLLLDL-FNTQIEMCDALTDPDAQLQTLATRIEAQGFRPYVIPVGGSSALGAMGYVESALEIAQQCEEVVG------LSSVVVASGSAGTHAGLAVGLEHLMPDVELIGVTVSRSVAEQKP------KVIALQQAIAGQLALTATADIHLWDDYFAPGYGVPNDAGMEAVKLLASLEGVLLDPVYTGKAMAGLIDGISQKRFNDDGPILFIHTGGAPA--------

General information: TITO was launched using: RESULT: Template: 4D9E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1253 -32799 -26.18 -132.79 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -26.18 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.416

(partial model without unconserved sides chains): PDB file : Tito_4D9E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D9E-query.scw PDB file : Tito_Scwrl_4D9E.pdb: