Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMQILKQIQVPYSFAKRHGVLFRYDGDQVFIVRRQNTEKIALQEARRILGKPAHYQLCTEQEFNSLLSTSYAGDTGESQQVAAGLEDHPDLLSLADQVPETEDLMDQEDDAPIVRLINALLSEAIRVGASDIHIEAFEKKLSVRLRVDGQLREIVQ-PRRELAPLLVSRIKVMAKLDIAEKRVPQDGRISLRLAGREVD-VRVSTLPSSHGERVVMRLLDKQAGRLNMTHLGLMANDYERLTQLVHRPHGIILVTGPTGSGKTTTLYAALSDLND-NTRNILTAEDPIEYQLEG----IGQTQVNTKVDMTFARALKAMLRQDPDVVMVGEIRDLETAEIAVQASLTGHLVLSTLHTNTAIGAVTRLKDMGIEPF--------LLSSSLIGVVAQRLVRTLCPHCMTWREADTFEKQVFQHISHEPSLKLPEAQGCDQCSHLGFNGRTAIYEIVPIDEPMRRLIHGNAAEFELENHARQYSGSIRDDGLRKVLSGKTTLEEVLRVTNEASEA 2EWW Chain:A ((18-289)) -----------------------------------------------------------------------------------------------------------------------IIKEAIELGASDIHLTA---GAPPAVRIDGYIKFLKDFPR--LTPEDTQKLAYSVMSEKHRQKLEENGQVDFSFGVRGVGRFRANVFYQRGSVAAALRSLPAEIPEFK--KLGLP----DKVLELCHRKMGLILVTGPTGSGKSTTIASMIDYINQTKSYHIITIEDPIEYVFKHKKSIVNQREVGEDTK-SFADALRAALREDPDVIFVGEMRDLETVETALRAAETGHLVFGTLHTNTAIDTIHRIVD--IFPLNQQEQVRIVLSFILQGIISQRLL------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2EWW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1136 2002 1.76 8.01 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 1.76 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_2EWW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EWW-query.scw PDB file : Tito_Scwrl_2EWW.pdb: