Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNTYIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITAFPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQILYQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGITRSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY 5EY1 Chain:A ((18-272)) -MSLLSKTRELNTLLQKHKGIAVD----FKDVAQTISSVTVTNVFIVSRRGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPENRELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHSEVEKEARDKAAITMAINSLSYSEKEAIEHIFEELG--EGLLI---------ITRSVIVNALRKLESAGVIESR------TFIKVKKEKFLDELEK---

General information: TITO was launched using: RESULT: Template: 5EY1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1004 -119342 -118.87 -501.44 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -118.87 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_5EY1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EY1-query.scw PDB file : Tito_Scwrl_5EY1.pdb: