Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPVVHAFRASQSGLDAGAMKRSSDSHSPPFFFLSSACLAYRTSPMPSPRFARPELNGIITVRRFQRPQLETEAPIRKGDLKEPLEAVAGRVGLRARCSTSAPSADLCLVDDHELVLMGKLVDKGVYYITGGVCIDAEGSDTSSQSPTAALVSSTACGAAPQPPDITVGWCFDARMLLHRSDMN-RSPETPHRLQRAIETLQGCERALDVLPVELLAPFTVHKDSSSGSDLGRAVPPISAANRSQWIPARLATYDEVCSFQDPQVYEHFLKSGAAL----VNLKSD----VYCNDETSSVAVRLSAGAVIDASVAALRGVAASRSGTASSAAGRVHPLVSFCLVRPPGHHCTASQPSGFCLVNNVAIAVQQLRIRHASALPSGPPRIAILDLDVHFGEGTASFVEGACDPASLLYLSLHRYDKRQFYPFDGRGDTAYVGGSRHAASKGSICNVAVHTNGQQPARCEQVISDHLMNSVLEEIFLPRLAEFGPDLVMVSLGFDAAYGDPLGKMAVE-GGFASVLSRLKWWCLHDGRTAGLVVVLEGGYNPEAVAQGV---LSVALALSLPRTDPL----------LQRFLEEKSPKVWSDLRQRQERRHREWQQLREERAEEDVGAAPSGLGSELKPAASDEPEQVQEDALLLDRHKRWCAALVAKVQQIHREAMTRER 5EEM Chain:B ((8-363)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------P-----ITGLVYDQRMMLHHNMWDSHHPELPQRISRI---------------------FSRHEE-------------LRLLSRCHRIPARLATEEELALCHSSK-HISIIKSSEHMKPRDLNRLGDEYNSIFISNESYTCAL-LAAGSCFNSAQAILTGQV--RNAVA--------------IVRPPGHHAEKDTACGFCFFNTAA-----LTARYAQSITRESLRVLIVDWDVHHGNGTQHIFE---EDDSVLYISLHRYEDGAFFPNSEDANYDKVG-----LGKGRGYNVNIPWNGGKMGDPEYMAAFH-------HLVMPIAREFAPELVLVSAGFDAARGDPLGGFQVTPEGYAHLTHQLM--SLAAGR---VLIILEGGYNLTSISESMSMCTSMLLGDSPPS---LTPLKTSATVSINNVLRAHAP-FWSSL------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5EEM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2136 -35887 -16.80 -108.75 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -16.80 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_5EEM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EEM-query.scw PDB file : Tito_Scwrl_5EEM.pdb: