Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASLQSRLAPITNINNNAYTTTAGAPILRKPEMTSAASAVYISRKEAAVSSSHGAPTNKMMATAAVTGSSRCEEATGRRSGSKRDHETSVTTDQNPQDAAKSLPPPPKKKKVTYALPHQNMEEGHFYVVLGEDIDVSTQRFKILSLLGEGTFGKVVESWDRKRK-EYCAVKIVRNVPKYTRDAKIEIQFMEKVRQADPADRFPLMKIQRYFQNDSGHMCIVMPKYGPCLLDWIMKHG--PFNHRHLAQIVFQTGVALDYFHSELHLMHTDLKPENILMETSDTT--VDPATNRH----LPPDPCRVRICDLGGCCDERHSRTAIVSTRHYRSPEVILGLGWMYSTDMWSMGCIIYELYTGKLLYDTHDNLEHLHLMEKTLGRLPSEWAARCGTEEA----RLLYN---SAGQL--RPCTDPKHLARIARARTVRDVIRDDLLCDLIYGLLHYDRQKRLNARQMTTHPYVLKYYPEARQAPSYPDNRAMLRPPPIM 5J1W Chain:A ((10-331)) --------------------------------------------------------------------------------------------------------------------------------------DVLSARYEIVDTLGEGAFGKVVECIDHKAGGRHVAVKIVKNVDRYCEAARSEIQVLEHLNTTDPNSTFRCVQMLEWFEH-HGHICIVFELLGLSTYDFIKENGFLPFRLDHIRKMAYQICKSVNFLHSN-KLTHTDLKPENILFVQSDYTEAYNPKIKRDERTLINPD---IKVVDFGSATYDDEHHSTLVSTRHYRAPEVILALGWSQPCDVWSIGCILIEYYLGFTVFPTHDSKEHLAMMERILGPLPKHMIQKTRKRKYFHHDRLDWDEHSSAGRYVSRACKPLKEF-------MLSQDVEHERLFDLIQKMLEYDPAKRITLREALKHPF---------------------------

General information: TITO was launched using: RESULT: Template: 5J1W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1449 -42647 -29.43 -140.29 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -29.43 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.535

(partial model without unconserved sides chains): PDB file : Tito_5J1W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5J1W-query.scw PDB file : Tito_Scwrl_5J1W.pdb: