Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence FVNTTESCEVERLFKATSPRGEPQDGLYCIRNSSTKSGKVLVVWDETSNKVRNYRIFEKDSKFYLEGEVLFVSVGSMVEHY 2RSY Chain:A ((99-156)) ----------------------PETGLFLVRESTNYPGDYTLCVS-CEGKVEHYRIMYHASKLSIDEEVYFENLMQLVEHY

General information: TITO was launched using: RESULT: Template: 2RSY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 176 -33060 -187.84 -569.99 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -187.84 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.555

(partial model without unconserved sides chains): PDB file : Tito_2RSY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RSY-query.scw PDB file : Tito_Scwrl_2RSY.pdb: