Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WFFRSQGRKEAERQLLAPINKAGSFLIRESETNKGAFSLSVKDVT-TQGELIKHYKIRCLDEGGYYISPRITFPSLQALVQHY 1BHF Chain:A ((9-91)) WFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHELVRHY

General information: TITO was launched using: RESULT: Template: 1BHF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 292 -13397 -45.88 -163.37 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.93 3D Compatibility (PKB) : -45.88 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.93 QMean score : 0.648

(partial model without unconserved sides chains): PDB file : Tito_1BHF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BHF-query.scw PDB file : Tito_Scwrl_1BHF.pdb: