Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WNDG---GFVNKQQAHDLLINKPDGTFLLRFSD-SEIGGITIAW--KFDSPERNLWNLKPFTTRDFSIRSLADRLGDLSYLIYVF 4E68 Chain:A ((454-539)) WNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGSTQIQSVEPYTKQQLNNMSFAE------------

General information: TITO was launched using: RESULT: Template: 4E68.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 198 13729 69.34 204.90 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 69.34 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.728

(partial model without unconserved sides chains): PDB file : Tito_4E68.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4E68-query.scw PDB file : Tito_Scwrl_4E68.pdb: