Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIHFSINKNLFLQALNITKRAISSKNAIPILSTVKIDVTNEGVTLIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHF-VLSQHKELKTVATDSHRLSQKKLTLEKNSDDFD--VVIPSRSLREFS--AVFTDDIE---TVEIFFANNQILFRSENISFYT-RLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVRPFTLVPADTDE-----------DFMQLITPVRTN 5AGU Chain:A ((17-403)) -LTFRLLRESFADAVSWVAKNLP------VLSGVLLTGSDNGLTISGFDYEVSAEAQVGAE-------IVSPGSVLVSGRLLSDITRALPNKPVDV-HVEGNRVALTCGNARFSLPTMPVEDYPTLPTLPEETGL-LPAELFAEAISQVAIAAGRDDTLPMLTGIRVEILGETVVL--AATDRFRLAVRELKWSASSPDIEAAVLVPAKTLAEAAKAGIGGSDVRLSLGTGPGVGKDGLLGISGNGKRSTTRLLDAEFPKFRQLLPTEHTAVATMDVAELIEAIKLVALV--A---AQVRMEFADGSVRLSAGADDVGRAEEDLVVDY-AGEPLTIAFNPTYLTDGLSSLRSERVSFGFTTAGKPALLRPVSG--NGNGPFPAVSTDYVYLLMPVR--

General information: TITO was launched using: RESULT: Template: 5AGU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1882 -96087 -51.06 -274.53 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -51.06 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_5AGU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AGU-query.scw PDB file : Tito_Scwrl_5AGU.pdb: