Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIRGFMGPPLE--SSFAT---C------LSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSS-SYPLYITTTKNTSTAQDMAKNLEIHHFFDGIYG--SSPE-APHKADVIHQALQTHQLVPQQAIIIGDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ 2HSZ Chain:A ((21-231)) -TQFKLIGFDLDGTLVNSLPDLALSINSALKDVNLPQASENLVMTWIGNGADVLSQRAVDWACKQAEKELTEDEFKYFKRQFGFYYGENLCNISRLYPNVKETLEALKAQGYILAVVTNKPTKHVQPILTAFGIDHLFSEMLGGQSLPEIKPHPAP-FYYLCGKFGLYPKQILFVGDSQNDIFAAHSAGCAVVGLTYGYNYNIPIAQSKPDWI------------

General information: TITO was launched using: RESULT: Template: 2HSZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1020 -50605 -49.61 -258.19 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -49.61 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_2HSZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HSZ-query.scw PDB file : Tito_Scwrl_2HSZ.pdb: