Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLMERNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNEHNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASISGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEVPCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLATTPTGRRLQKEIFGE 5C1J Chain:A ((58-131)) -------------------------------------------------------------------------------------------------------------------------------------------------------------ASFMGAEAAKEAVRDAGLKLEDIDLIINASGSPEQAVPDGGPLVQRELGL------GRSGVPSITVNASCLSF-FVALDVA----------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5C1J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 262 -23552 -89.89 -318.26 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : -89.89 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.097

(partial model without unconserved sides chains): PDB file : Tito_5C1J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C1J-query.scw PDB file : Tito_Scwrl_5C1J.pdb: