Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQDNYTTKGKHLTID----SRRLIERWKKEGKSN-REVASLLGKAPQTIHTEIKR----GTVRKCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYT 2ZNY Chain:A ((25-73)) ---------------LDEIDKKIIKILQNDGKAPLREISKITGLAESTIHERIRKLRESGVIKK---------------------------------------------

General information: TITO was launched using: RESULT: Template: 2ZNY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 90 4376 48.62 109.40 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 48.62 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.870

(partial model without unconserved sides chains): PDB file : Tito_2ZNY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZNY-query.scw PDB file : Tito_Scwrl_2ZNY.pdb: