Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGHVENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVI-CYKATEFSGTLQSSEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF 2RRK Chain:A ((21-132)) ---------------------KILLAQRPAQSDQAGLWEFAGGKVEPDESQRQALVRELREELGIEATVGEYVASHQ--REVSGRIIHLHAWHVPDFHGTLQAHEHQALVWCSPEEALQYPLAPADIPLLEAFMA---------------------

General information: TITO was launched using: RESULT: Template: 2RRK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 459 -26847 -58.49 -241.86 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -58.49 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.377

(partial model without unconserved sides chains): PDB file : Tito_2RRK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RRK-query.scw PDB file : Tito_Scwrl_2RRK.pdb: