Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEPSIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPLTPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPNRAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILT--DACKIFVIKGKDVALAKGEYSNLKITTVTDLKIAKSMIEKD 5HS2 Chain:A ((7-226)) -----IPAAGQGKRMK-AGRNKLFIELKGDPVIIHTLRVFDSHRQCDKIILVINEQEREHFQQLLSDY-P-FQTSIELVAGGDERQHSVYKGLKAV------KQEKIVLVHDGARPFIKHEQIDELIAEAEQTGAAILAVPVKDTIKRVQDLQ-VSETIERSSLWAVQTPQAFRLSLLMKAHAEA--ERKGFLGTDDASLVEQMEGGSVRVVEGSYTNIKLTTPDDLTSAEAIMESE

General information: TITO was launched using: RESULT: Template: 5HS2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1038 -84880 -81.77 -389.36 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -81.77 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.410

(partial model without unconserved sides chains): PDB file : Tito_5HS2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HS2-query.scw PDB file : Tito_Scwrl_5HS2.pdb: