Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRRELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSKNAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAESKEDAA 5GAF Chain:I ((8-115)) --------KQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKAGREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYVTEHPGAAARLFKEFAKANAKFEVKAAAFEG--------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5GAF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain I - contact count / total energy / energy per contact / energy per residue : 472 -64921 -137.54 -601.12 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain I : 0.70 3D Compatibility (PKB) : -137.54 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.660

(partial model without unconserved sides chains): PDB file : Tito_5GAF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GAF-query.scw PDB file : Tito_Scwrl_5GAF.pdb: