Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSDIENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRTVIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLTRFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGEN-RVESVVFENVKTGQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLKDLRQVTTAVGDGAIAGQEAYKFITEHS 5MIS Chain:A ((25-325)) -YDVVIIGSGPAGMTAAMYTARSEMKTLLLERGVPGGQMNNTAEIENYPGYETIMGPELSMKMAEPLEGLGVENAYGFVTGIEDHGDYKKIITEDDEFITKSIIIATGANHRKLEIPGEEEYGARGVSYCAVCDGAFFRNQEILVIGGGDSAVEEALYLTRFGQSVTIMHRRDKLRAQEIIQQRAFKEEKINFIWDSVPMEIKGDDKKIQSVVYKNVKTGEVTEKAFGGIFIYVGLDPVAEFVSDLGITDEAGWIITDDHMRTNIPGIFAVGDVRQKDFRQITTAVGDGAQAAQEAYKFVVE--

General information: TITO was launched using: RESULT: Template: 5MIS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1822 -115905 -63.61 -386.35 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.94 3D Compatibility (PKB) : -63.61 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.539

(partial model without unconserved sides chains): PDB file : Tito_5MIS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MIS-query.scw PDB file : Tito_Scwrl_5MIS.pdb: