Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIDTQLTTLFCLIDDFCTDITQNVEQYMLTYGQTKRLRQSKIHASEVITLLLWFHLTGSRNFKAFYLYWAKPFLCSYFTNLPSYSRFIELKAKYAMYFVALIESLKVHSAGIAFIDSTKLAVCHNKRIHQHRVFADSASRGKTSVDWFYGFKIHLICDHIGRLVSYCITTGNVDDRKVLPDLIEHSKLKGKLFGDRGYVGKNWKSRLAEVGVQLITRVKRNMKPQVLAPFDHAVLKKRGIIEAPFKLMKSQFDLEHSRHRSKMGLLTTIFAALTLYALVLVNGYKSGIQQILKPIDLNSA 1OGD Chain:B ((22-36)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DKIVIADAGLPVPDG-------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1OGD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 6 -1554 -258.92 -103.57 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.48 3D Compatibility (PKB) : -258.92 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.48 QMean score : 0.564

(partial model without unconserved sides chains): PDB file : Tito_1OGD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1OGD-query.scw PDB file : Tito_Scwrl_1OGD.pdb: