Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTPEDISQNKPLLWLMAAACGLCAGVNYYCQPLIHSIQQDFAVTQAQAALTVTFAQVSYALGLLFIVPMGDIVNKAKAIPFLMMLCAVGLFTSAFAVNLPMLWIGTILAGLFS-VAAQVLIPLATMTVKPEKTGEIIGFLMSGLLVGILLSTSLAGLFSNLFHWKVVYAISGILMLLLAYTLKSRLPYVMRMKLNYGQIFVSMAQLLKEEKRLLLRALTGAFAFASVSILYSTIALLMTSAHHLPDLFIGLV-PLVGIFGALSTRYIGKYADQGYTRLLTWMGCGLFLLSWICFYF-G-QTLLSAYVIGFALIQLALALVHTSNQSIIFRLRPDAKSRINAIYMTTYFIGGAAGSALGIFAWNR--GEWTMTCLAGVGLVVFCILFSMIDAFLQKKQMA 2GFP Chain:A ((16-378)) -------------------LVAVGQMAQTIYIPAIADMARDLNVREGAVQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAASAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWNWRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTR-LLTSYKTLFGNSGFNCYLLMLIGGLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQSVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGTAGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVL-----------------

General information: TITO was launched using: RESULT: Template: 2GFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1808 -297047 -164.30 -832.06 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -164.30 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.240

(partial model without unconserved sides chains): PDB file : Tito_2GFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GFP-query.scw PDB file : Tito_Scwrl_2GFP.pdb: