Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLDYLQALKRIPEARGAWILHGQEPLLEQNLLDTFRKSWQQQDIERQRYDISSVSDWKNVFNALNSLSLF-SQQLAIEVHGNIKPDANGLKQLKSYIQHNETNLLLIVLPKQDSSSLKSAFFQVVEANGVVVALTANYPQDRQ--RILAAEAEKLEIQLDNDAWQWLMQHHEHNLLAAKNSLMRVRDTFPDQKLIQIEQLYACLQDQSRYTTYDLSDALLEGNLAQSIKIFQYLIGSGEPESLILWTLSKEMRLLMQLFEQ----PHNAL--QLGIWKTKVSLYQQALRRLNPQQFLGWSALLLQIDAAIKGMSNENAQHLMQQAIAELCGKTLFIH 3GLH Chain:A ((49-331)) ---------------------------------------------EHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTGLLHDDLLLIVRGNKLSKAQENAAWFTALANRSV-QVTCQ-PEQAQLPRWVAARAKQLNLELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLT-LPRVEQAVNDAA-FTPFHWVDALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKHRVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLK-------QDYGQSVWAELEGLSLLL-

General information: TITO was launched using: RESULT: Template: 3GLH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 930 -29988 -32.24 -110.25 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -32.24 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.489

(partial model without unconserved sides chains): PDB file : Tito_3GLH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GLH-query.scw PDB file : Tito_Scwrl_3GLH.pdb: