Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAANVIVYSTSVCPYCVRAKQLLERKGVAYKEVNLSVEAPEVRAELMQRTNHRTVPQIFINDQFIGGFDQLYALEREGKLDELLA 4TR0 Chain:A ((3-83)) ---NITIYTKNYCPYCKKAVSLLSSKGVDFKEVDVTHDSKAFE-DVMAKTGWDTVPQVFVDEEFLGGCDDIHALDRQGILDKKLG

General information: TITO was launched using: RESULT: Template: 4TR0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 327 -56170 -171.77 -693.45 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -171.77 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.829

(partial model without unconserved sides chains): PDB file : Tito_4TR0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TR0-query.scw PDB file : Tito_Scwrl_4TR0.pdb: