Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVRINKMNGKHVDIYIGKLNDFLNRKQHDFPDFRTFNEYKKQQIQLIKNKLIAQKLEIAPTDLVFAKHEYGKPYLLDHALHFNHSHSQQYYALALSERVKDIGIDVEEL----DRKVRLDSLAQHAFHPDEYATWQSLEQDR--EYWFKVWTTKEAVLKASGLGIR----LDLNTLNTQAPPTNHGGLCSHELIGTFAYQNFVLGNMILTVAWRSEQSCRGFQFPSIQIHSLDR 1FTE Chain:A ((8-84)) ------------------------------------------------------------------------------------------------------GIDIEELASIESAVTRHEGFAKRVLTALEMERFTSLKGRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNE-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1FTE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 167 7869 47.12 117.44 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 47.12 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_1FTE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FTE-query.scw PDB file : Tito_Scwrl_1FTE.pdb: