Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGALMLDIAGTELTQEDIELLQAPQVGGMILFARNIESPQQVRALTDHMRQVRPDILIAVDQEGGRVQRLKSGFTLLPAMGRFGELYITQPQKALELAEQCGWLMATEVLAVGIDFSFAPVLDLN-AISDVIGDRGFSKNIEDIAPLAGAFMRGMKKAGMANTGKHFPGHGSVKADSHVAAAIDSRSYDEIYNHDMQSFIKLMPELDALMPAHVIYDQVDLNPAGFSPFWIQEVLRNRLKFNGVLFSDDLSMQAACVAGGADARIQAALAAGCDMGLVCNDRSAACTALDGITNLELPNQERLERMRGRIPQIQVGETLSLGNDWQAVKTAIEEFKNSF 5G6T Chain:A ((21-325)) MQGSLMLDIGGTWLTAEDRQILRHPEVGGLIIFARNIEHPAQVRELCAAIRAIRPDLLLAVDQEGGRVQRLRQGFVRLPAM-----RAIADNPNAEELAEHCGWLMATEVQAVGLDLSFAPVLDLDHQ------------DPERAALLAGAFIRGMHAAGMAATGKHFPGHGWAE----VAIPEDARSLEEIRRSDLVPFARLAGQLDALMPAHVIYPQVDPQPAGFSRRWLQEILRGELKFDGVIFSDDLSMAGAHVVGDAASRIEAALAAGCDMGLVCNDRASAELALAALQRLKVTPPSRLQRMRGK------------------------------

General information: TITO was launched using: RESULT: Template: 5G6T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1611 -87899 -54.56 -305.20 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -54.56 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.459

(partial model without unconserved sides chains): PDB file : Tito_5G6T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5G6T-query.scw PDB file : Tito_Scwrl_5G6T.pdb: