Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDNATILQHVPVGKKVGIAFSGGLDTSAALLWM-KQKGAEPYAYTANLGQPDEDDYDAIPKKAEQYGAVKARLIDCRLQLALE-GIAAIQCGAFHISTGGVPYFNTTPLGRAVTGTMLVTAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPNLKIYKPWLDQNFIDELGGRAEMSQFLIDNGFDYKMSKEKAYSTDSNMLGATHEAKDLEYL-NAGIKIV-----DPIMGVAFWKEEVEIKPEEVTIRFEEGVPVALNGQRFDNPVELILEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQGRWFDSQALMLRETAQRWVAKA---ITGEVTLELRRGNDYTIMNTESPNLTYEAERLTMEKGDSMFTPMDRIGQLTMRNLDITDTRAKLGIYTDAGLLSIGQGSALPQLDNKKK 4U7J Chain:A ((11-381)) --------------ERVILAYSGGLDTSVAISWIGKETGREVVAVAIDLGQGGED-MEVVRQRALDCGAVESIVIDARDEFANDYCVPAIQSNALYMDR----YPLVSALSRPLIVKHLVKAAREHGGTIVAHGCTGKGNDQVRFEVGFASLAPDLEVLAPVRDYAWT-----REKAIAFAEENNIPINVTKRSPFSIDQNVWGRAVETGFLEHLWNAPTKDVYSYTEDPTVN---WS-----TPDEVIVGFEQGVPVSIDG-RSVTPLQAIEELNRRGGEQGVGRLDVVEDRLVGIKSREIYEAPGAMVLITAHTELEHVTLERELGRFKRITDQKWGELVYDGLWFSP----LKTALESFVAKTQEHVTGEIRMVL-HGGHIAVNGRRSPKSLYDFNLATYDEGDTF-------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4U7J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1971 -2262 -1.15 -6.28 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -1.15 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_4U7J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4U7J-query.scw PDB file : Tito_Scwrl_4U7J.pdb: