Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHTSTTSTVPLEPWIAQQLQQATQGYWHKDQIPQSEIKRILTDSRHAESGDAFLALKGERFDAHNFVAQVAANGCQVAIVERPIDAEIAQLVVADTRLALGQLGAYRREQNAQLKVIALTGSSGKTTTKEMLGSILSRLAPTLINRGNLNNDLGVPMMLLELRKEHQYAVMELGANHQGEIDYTSKIVQPHVAGILNIGTAHLGEFGGRDGICRAKSEIYRHILPQGVAIVPQQDDFTAEIR-EAAKSHQIMSFGA-GGDVFATEIELLPQS--ANFQLHTPQGSSFVRLPFAGEHN---VQNATAAVAFALALGVSLEDIVKGLEQAQGAKGRLNFIQKAPHLFIDDTYNANPTSMRAAAQVLLQQNGIKVMVMGDIGELGDSSWQEHHDLGRDLAGLPLDHIVAVGQFASAALEGAGLHSTKLKAFQTQAEALPFLINLIQTHQPQSMSFLFKGSRFTHMETLMADLMEKL 3ZL8 Chain:A ((7-355)) -------------------------------------RRFVLNSKEVREGDVFVAVKGKRFDGHDFIDEALRNGAYAIIAERKTVNSDRIFLVESSVDTLAKL-AREKLGNFSGTVVGVTGSSGKTTTKEILYNLLKNKRSVFKTPGNMNTEYGLPLSILNDYKGEEILVLEMAASRPGDIAHLCKIAPPDVAVLLNVGSAHLEFFGTRERIMETKMEIIKHSKENAIAVTLFDD---PDLRKEVPRYRNTLFFGKEGGDSVLKDWWYYEGSTIAEFEAF----DSLFTVKLSGYWNGGQLLNIAASLCVMRTLGETVD--IFDLASLKTVPGRFNVREKKGVLIVDDTYNASPEAFQTSIEALLRFPGKKFAVVGAMKELGERSKEFHEELGERL-----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3ZL8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1917 -136757 -71.34 -399.87 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -71.34 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.495

(partial model without unconserved sides chains): PDB file : Tito_3ZL8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZL8-query.scw PDB file : Tito_Scwrl_3ZL8.pdb: