Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREIILISFLGPDQPNQFTRLMQVLSVHSLQILDVGQAVIHNQLTLGIVVASENETATALAMKEILILAHDIGLTVRFK--PISGAEYDQWVSEGGRTRYIVTALAPELTAAHLQAVTQIVSSQGFNIETVTRLSGR--VDLEKDSTLPRRACVQFGLSSGPTLDAQAMRAACLLLSSELNIDVAVQEDNAYRRNRRLVCFDMDSTLIEQEVIDELALEAGVGEQVAEITERAMQGELDFQQSFRARVALLKGLDASVLPKIAERLTITEGAERLISTLKVLGYKTAILSGGFQYFAEYLQAKLGIDEVHANVLDVQDGVVTGEVKGVIVDGARKAELLRELANKLGISLEQAMAVGDGANDLPMLAIAGLGVAYRAKPLVRQNANQAISSVGLDGVLYLLGMHDKDLSRA 5IT4 Chain:A ((5-384)) ----VLITVTGVDQPGVTATLFEVLSRHGVELLNVEQVVIRHRLTLGVLVCCPADVADGPALR------HDVEAAIRKVGLDVSIERSD-VPIIREPSTHTIFVLGRPITAAAFGAVAREVAALGVNIDLIRGVSDYPVIGLELRVSVP------------PGADG-ALRTALNRVSSEEHVDVAVEDYTLERRAKRLIVFDVDSTLVQGEVIEMLAAKAGAEGQVAAITDAAMRGELDFAQSLQQRVATLAGLPATVIDEVAGQLELMPGARTTLRTLRRLGYACGVVSGGFRRIIEPLAEELMLDYVAANELEIVDGTLTGRVVGPIIDRAGKATALREFAQRAGVPMAQTVAVGNGANDIDMLAAAGLGIAFNAKPALREVADASLSHPYLDTVLFLLGV--------

General information: TITO was launched using: RESULT: Template: 5IT4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2020 -108145 -53.54 -288.39 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -53.54 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.543

(partial model without unconserved sides chains): PDB file : Tito_5IT4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IT4-query.scw PDB file : Tito_Scwrl_5IT4.pdb: