Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDTRENWTSRSGFIIAAVGSAVGLGNIWRFPYVAYENGGGAFLIPYLLALITAGLPLLFLDYAVG-HRSTGSPPKAYR--ALFKGGETLGWWQVCVCIIIGLYYASVLTWA---------GSYVYFSIGQMWGSDP---------EGF------FFNTY-LQTTKATGFDLQFVGHLFWPIVGIWALTLIILYGGVKKGVELSNKI--FMPLL-FILFTILVIQSLRLPGAVQGLNAFFTPNWSAMMDYKVWLAAYGHTFFSLSVGFGIMVTYASYLKPKTNLTGSGLIVGFANASTEILAGIGIFAALGFMAHAAGTEVKDVVSGGIGLAFIAFPKIISSLGSGADLFGLLFFSSLFVAGISSMVSILEVPIAAMQDKLKWGRKKAVTIIGGGSALVSIILFSSVNA--IKLVDIVDHFINNIGIIGGALLSIISVAWFKRSALKELRDHVNRISTIQLGKGWDFTLTVITSLILLTTLCMTVFNLIKNGYDTYS----MSLQGVFGW----GSVIFCAVVAIVLSKMKDR 4XPT Chain:A ((1-512)) --DERETWSGKVDFLLSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYGIMLAVGGIPLFYMELALGQHNRKGAITCWGRLVPLFKG---IGYAVVLIAFYVGFYYNVIIAWSLRFFFASFTNSLPWTSCNNIWNTPNCRPFEVGHVEGFQSAASEYFNRYILELNRSEG--IHDLGAIKWDMALCLLIVYLICYFSLWKGISTSGKVVWFTALFPYAVLLILLIRGLTLPGSFLGIQYYLTPNFSAIYKAEVWVDAATQVFFSLGPGFGVLLAYASYNKYHNNVYKDALLTSFINSATSFIAGFVIFSVLGYMAHTLGVRIEDVATEGPGLVFVVYPAAIATM-PASTFWALIFFMMLATLGLDSSFGGMEAIITALSDEFPKIKRNRELFVAGLFSLYFVVGLASCTQGGFYFFHLLDRYAAGYSILVAVFFEAIAVSWIYGT--NRFSEDIRDMIGFPPGRYWQVCWRFVAPIFLLF---ITVYGLIGYEPLTYADYVYPSWANALGWCIAGSSVVMIPAVAI--------

General information: TITO was launched using: RESULT: Template: 4XPT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2652 -339197 -127.90 -720.16 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -127.90 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.369

(partial model without unconserved sides chains): PDB file : Tito_4XPT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XPT-query.scw PDB file : Tito_Scwrl_4XPT.pdb: