Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPELPEVETTKTSLFPLLNQKVL-SVEVRNPSL-RWPIPDNV--QKLVGQRLIGLNRRSKYILAEFEQDQMLWHLGMSGSFRLCQPNDELRKHDHLIIQFED-QQLRYHDPRRFGCILWLNPETQGKL--IDTLGPEPLSTDFHAEYLASKLKNKSVGIKIALMDNHVVVGVGNIYATESLFNVGIHPAQPAGDLTMQQIEKLVVEIKRILKSAIDLGGSTLRDYSNAMGENGYFQQTLLAYGRAREMCVNCETTLENLKLGQRASVFCPQCQPLKKLRKP 1L2D Chain:A ((2-273)) -PELPEVETIRRTLLPLIVGKTIEDVRIFWPNIIRHPRDSEAFAARMIGQTVRGLERRGKFLKFLLDRDALISHLRMEGRYAVASALEPLEPHTHVVFCFTDGSELRYRDVRKFGTMHVYAKEEADRRPPLAELGPEPLSPAFSPAVLAERAVKTKRSVKALLLDQTVVAGFGNIYVDESLFRAGILPGRPAASLSSKEIERLHEEMVATIGEAVMKGGS--------------FQHHLYVYGRQGNPCKRCGTPIEKTVVAGRGTHYCPRCQ--------

General information: TITO was launched using: RESULT: Template: 1L2D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1231 18672 15.17 74.39 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : 15.17 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.375

(partial model without unconserved sides chains): PDB file : Tito_1L2D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1L2D-query.scw PDB file : Tito_Scwrl_1L2D.pdb: