Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDSNMEVLIKTEDAIDAYNGAANLARSLGVSRAAISQ-----WGEY----VPEVSARGLVILQPSIPHIIKKPSAT 2W7N Chain:A ((38-91)) ---------------------ATFATSLGLTRGAVSQAVHRVWAAFEDKNLPEGYARVTAVLPEHQAYIVRKWEA-

General information: TITO was launched using: RESULT: Template: 2W7N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 35 -7705 -220.13 -171.21 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -220.13 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_2W7N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W7N-query.scw PDB file : Tito_Scwrl_2W7N.pdb: