Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKEFLKAYVQTFKDIVSNSSAFTTLFLSVILYSFFYPTAYKAERAESIPIVIVDEEQSLLTSQVIGQTANSPHVKIVDVTANFLEAEQMVREQ---KADGILLLPSNLTQSLRRGETGGIGLYLSTTNFLKTKEIGLGLATSIEATLKEYIERFGQRTHFQPALSIHQMPLFNTLSGYGSYIFPAVASLIIHQTIVLGLAMLVASYREQHEKITPIRFAGIFASIFTIGCLGSFYLFGFTLWFNDYPHGGNFVGLLVAVPIFISCVIGLGMLIGSLLDILERAGHIIVFSSVPLFLLTGAAWPHQAMPEWLQWFAWCLPSTHAVQMFVQLNQMGVPLNVVAPKLIFLATIGVIFLITAYSRLKVSK 4YTZ Chain:A ((718-773)) -----------------------------------------------------------------------------DLKKGFSEADNVVSGELYIGGQEHFYLETNCTIAVPKGEAGEMELFVSTQNTMKTQ-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4YTZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 43 2716 63.16 51.25 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.44 3D Compatibility (PKB) : 63.16 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.44 QMean score : 0.321

(partial model without unconserved sides chains): PDB file : Tito_4YTZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YTZ-query.scw PDB file : Tito_Scwrl_4YTZ.pdb: