Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNKNIGCWLLLAGSSSVCAMQPLDDQSLAAATGQNGLTLGIQADQVKFNQVALIDTNGIASTSYNSKAGLIIAGNSTNPVPGIEFIKAAVSTNPSFNIAIDTDAGGGNPFLNLAVTMGSDVNGIRLLPFSVYLAPSTSLPSPSDYALTSYAPKSIFSSGTTVNTGVKELIRSTGNLDINFVQTNKPRLNIQLGHAAQSVMVKFGGAIQSICSAASGCPITLVSDNTGATFGFKFAGTNASTGFVLDGFYAGVDPTGLTIGNIGVSSKFDASLNNVTLGNLGTQSTTTFNNLPNGSMGSFGVTGASVTDFKMKVSGF 3N27 Chain:B ((28-58)) ------------TAEKTVYVLTALQDY----SGGSGGIDISIELNKA------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3N27.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 44 -381 -8.65 -12.27 target 2D structure prediction score : 0.29 Monomeric hydrophicity matching model chain B : 0.48 3D Compatibility (PKB) : -8.65 2D Compatibility (Sec. Struct. Predict.) : 0.29 1D Compatibility (Hydrophobicity) : 0.48 QMean score : 0.201

(partial model without unconserved sides chains): PDB file : Tito_3N27.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N27-query.scw PDB file : Tito_Scwrl_3N27.pdb: