Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPVMANYST-NDFKPGLKVMLDSNPCSIMENEYVKPGKGQAFNRVKLRNLKTGKVLEKTFKSGDTLEAADIVEVEMNYLYNDGEMWHFMDPESFEQIAADKTAMGDAAKWLKDDSNETCTIMLFNGVPLNVNAPNFVVLKVVETDPGVRGDTSGGGGKPAKLETGAVVRVPLFVQQEESVRVDTRTGEYLERA 3OYY Chain:A ((1-188)) ---MASMKTAQEFRAGQVANINGAPWVIQKAEFNKSGRNAAVVKMKLKNLLTGAGTETVFKADDKLEPIILDRKEVTYSYFADPLYVFMDSE-FNQYEIEKDDLEGVLTFIEDGMTDICEAVFYNDKVISVELPTTIVRQIAYTEPAVRGDTS--VMKTARLNNGAELQVSAFCEIGDSIEIDTRTGEYKSR-

General information: TITO was launched using: RESULT: Template: 3OYY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 772 47872 62.01 258.76 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 62.01 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_3OYY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OYY-query.scw PDB file : Tito_Scwrl_3OYY.pdb: