Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLSQFVNKTLKVLALCTGLISTSHFAQANDFVTHPNYLNFKQKAMSTYGLSGEQVDAAMNGAKNLPNIINIM-----TRPGESKPWYDYRSMFLVEGTIQRGVRFKNQYADALNRAEQQFGVSQAVILGILGVETGYGANKGSFITRDALATLAFGYPRRAEYFGDELAALIAWTYKEGYPTNSIVGSYAGAIGFPQFMPSNISKYGVDFDGNGHIDLRNSAEDAIGSIANYLAKQGWQCDQPIGFIARYTGSNPESIIAKDLTQPTPYGALKNQGISPLNPLVKIDDLDLVNVIQLQDYNGPIYYLTYPNFQVITTYNKSRMYATAVWLLGTEVASR 1D0K Chain:A ((14-317)) -----------------------------DFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAP---GPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIGVETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFAGAMGYGQFMPSSYKQYAVDFSGDGHINLWDPV-DAIGSVANYFKAHGWVKGDQVAVMA--NGQAPG--LPNGFKTKYSISQLAAAGLTPQQPL---GNHQQASLLRLDVGTGYQYWYGLPNFYTITRYNHSTHYAMAVWQLGQAVA--

General information: TITO was launched using: RESULT: Template: 1D0K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1495 -16085 -10.76 -55.27 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -10.76 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.573

(partial model without unconserved sides chains): PDB file : Tito_1D0K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1D0K-query.scw PDB file : Tito_Scwrl_1D0K.pdb: