Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFNQLQIALGTLVRKEVRRFLRIWPQTLLPPAITMSLYFVIFGNLVGSRIGEMGGVSYMQFIVPGLIMM---AVITNSYANVSSSFFSVKFQKNIEELIMSPVPLHIILWGYVIGGICRGVLVGAIVTAMSMFFTDLFI--HNWFVTIYTVLITSVLFSLGGFINAVYAKSFDDISIIPTFVLTPLTYL-GGVFYAISALGPFWQKLSLINPIVYMVNAFRFGILGHSD-V-------------------NVSFSLIIVTLCCAVLYGIAYYLLARGSGMRE 5DO7 Chain:A ((362-649)) ---PGVFSKLGVLLRRVTRNLVRNKL-AVITRLLQNLIMGLFLLFFVLRVRS--NVLKGAIQDRVGLLYQFVGATPYTGM-LNAVNLFPVLRAVSDQESQDGLYQKWQMMLAYALHVLPFSVVATMIFSSVCYWTLGLHPEVARFGYFSAALLAPHLIGEFLTLVLLGIVQNPNIVNSVVALLSIAGVLVGSGFLRNIQEMPIPFKIISYFTFQKYCSEILVVNEFYGLNFTCAFTQGIQFIEKTCPGATSRFTMNFLILYSFIPALVILGIVVFKIRDHLI-

General information: TITO was launched using: RESULT: Template: 5DO7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1153 -176297 -152.90 -708.02 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -152.90 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.370

(partial model without unconserved sides chains): PDB file : Tito_5DO7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DO7-query.scw PDB file : Tito_Scwrl_5DO7.pdb: