Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITANKGEWSEVYALFKLLSEGKLYAGDKDLNKIPNLIYPIISILRQESQDLLTYSPEPNHGVVHIQNGTSTFTISQSDFKGITELLLTEIKKKQPTASFSIPEVETFISQYNSKKIKAKSSAKSDIRIIIYDQKIGTTPELGFSIKSKLGKASTLLNASQATNFVYKVKNLTLSPQEIDDFNQLEFTVPIIQGRIHHLESLGGSVEFSHITNDVFNNNLILLDSLLPNIISEALYKYYTSPITSVKEIFEQVASANPIHYSLQHQHPFYEYKVKKLLCEIAIGMMPSTVWTGNNIDATGGYLVIKEDGDVICYHLYHRHEFEEYLYNNTRFEAASKSRHNYGNLFIENGELYMTLNLQIRFK 5C6B Chain:F ((128-174)) -----------------------------------------------------------NKAVVSLSNGVSVLTSKVLDLKNYIDKQLLPIVNKQ---SCSIPNIETVI--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5C6B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 66 -1868 -28.30 -39.74 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain F : 0.53 3D Compatibility (PKB) : -28.30 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.460

(partial model without unconserved sides chains): PDB file : Tito_5C6B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C6B-query.scw PDB file : Tito_Scwrl_5C6B.pdb: