Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIIGYARTTINDVDLTTQVDQLSDFGCQ--EIFHETYEVEQNDSTAVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTRE-MGKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRP-KIDAKTIKKIRRLYYEKKETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI 1ZR4 Chain:A ((1-180)) MALFGYARVSTSQQSLDIQVRALKDAGVKANRIFTDKASGSSSDRKGLDLLRM--KVKEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVSIRFIDDGISTDSYIGKMVVTILSAVAQAERQRILQRTNEGRQEAMAKGVVFGRKRKIDRDAVLNM----WQQGLGASHISKTMNIARSTVYKVIN-----------

General information: TITO was launched using: RESULT: Template: 1ZR4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 559 -15126 -27.06 -85.94 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -27.06 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.464

(partial model without unconserved sides chains): PDB file : Tito_1ZR4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZR4-query.scw PDB file : Tito_Scwrl_1ZR4.pdb: